Abstract– Mesenchymal stem cell (MSC) and conditioned medium-MSC (CM-MSC) are potential

therapeutic agents for the treatment of neurogenerative diseases. Conditioned medium-mesenchymal stem

cell (CM-MSC) contained growth factors that can protect neuronal cells from cell death and induce

differentiation of neural stem/progenitor cells (NPCs). Aims : To investigate the capability of conditioned

medium rat bone marrow-derived mesenchymal stem cells (CM-rat BMMSC) induces in vitro differentiation

of NPCs into astrocytes and neuron. Methods : We assessed the effects of CM-ratBMMSC to induce

differentiation of NPCs by culturing cells in serum-free medium DMEM/F12 (FM), CM 100%, CM 50% and

neuro basal medium with supplement (NM) for 4 days. We examined the effects on differentiation by

assessing the expression of A2B5, PSANCAM, and beta-tubulin by flowcytometry, and the expressions of

glial fibrilary acidicprotein (GFAP) and neuronal nuclei (NeuN) by immunocytochemistry (ICC). Results:

CM-ratBMMSC induced differentiation of NPC to astrocytes and neurons which indicated by the expression

of GFAP, beta-tubulin and NeuN. Our findings showed that growth factors or neurotrophic factors such as

basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) in CM-ratBMMSC induces NPCs

differentiations that may happen via phosphoinositide 3-kinases (PI3K)/protein or kinase B (Akt) signalling

pathway. Conclusions : Growth and neurotrophic factors from CM-rat BMMSC such as bFGF and NGF

increased the differentiation ability of NPCs into astrocytes (GFAP) and neurons (NeuN)